RT-PCR or Reverse Transcriptase PCR is a type of polymerase chain reaction that is used to measures levels of RNA expression present in the given sample. Modern DNA performs reverse osmosis of their RNA expressions using an enzyme called reverse transcriptase.
This technique can be used to measure the quality of genes and can be put back together with real-time PCR to measure RNA levels. RT-PCR kit is used in research labs to examine gene expression, in experiments like differentiating exons from introns, to diagnose hereditary diseases, and to supervise drug treatment. You can buy a high-quality reverse transcriptase PCR kit to perform them.
The RT-PCR kit requires an RNA template, an enzyme, nucleotides, buffers, and thermos-cyclers to generate RT-PCR solutions from the kit. Kits make the procedure simple. Otherwise, the procedure can be complicated since RNase, the enzyme which degrades RNA is present anywhere and everywhere, and the tiniest contamination of DNA can interrupt effects.
You can use RNase free consumables and reagents that include RNase inhibitors and RNA stabilizers that guarantees accurate and trustworthy RT-PCR result. RT-PCR is a common diagnostic method and is mostly used in combination with quantitative real-time PCR or qPCR which is widely used to weigh RNA transcript levels present in cells and tissues.
The combination of real-time PCR and reverse transcription PCR is called quantitative RT-PCR or qRT-PCR. All the reagents needed to perform reverse transcription with PCR are included in RT-PCR kits. You can choose the enhanced AMV kit to do different experiments where the transcripts are in low abundance or have complex secondary structures.